› 4kjd

X-ray diffraction
2.21Å resolution

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intestinal-type alkaline phosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 488 amino acids
Theoretical weight: 53.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P15693 (Residues: 21-502; Coverage: 93%)
Gene name: Alpi
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GOL 2 x GOL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 63.321Å b: 75.726Å c: 170.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.203
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Prodrug Approach: A Successful Tool for Improving Drug Solubility.
Jornada et al. (2015)
1 more

2 mentions without citation

Structural and Functional Characterization of the BcsG Subunit of the Cellulose Synthase in Salmonella typhimurium.
Sun et al. (2018)
1 more