› 4kq0

X-ray diffraction
2.1Å resolution

Crystal structure of double-helical CGG-repetitive RNA 19mer complexed with RSS p19

Released:
DOI: 10.2210/pdb4kq0/pdb
PDB entry 4kq0 coloured by chain, front view.
PDB entry 4kq0 coloured by chain, side view.
PDB entry 4kq0 coloured by chain, top view.
Source organism: Tomato bushy stunt virus
Entry authors: Cabo A, Katorcha E, Tamjar J, Popov AN, Malinina L

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159607 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA silencing suppressor p19 Chains: A, D
Molecule details ›
Chains: A, D
Length: 135 amino acids
Theoretical weight: 15.41 KDa
Source organism: Tomato bushy stunt virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69517 (Residues: 27-157; Coverage: 76%)
Gene name: ORF4
Sequence domains: Tombusvirus P19 core protein
Structure domains: RNA silencing suppressor P19
5'-R(P*GP*GP*CP*GP*GP*CP*GP*GP*CP*GP*GP*CP*GP*GP*CP*GP*GP*CP*C)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 19 nucleotides
Theoretical weight: 6.23 KDa
Source organism: Tomato bushy stunt virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R3
Unit cell:
a: 90.926Å b: 90.926Å c: 147.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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