› 4kug

X-ray diffraction
2.3Å resolution

Crystal structure of 3-hydroxybutylryl-CoA dehydrogenase with NAD from Clostridium butyricum

Released:
DOI: 10.2210/pdb4kug/pdb
PDB entry 4kug coloured by chain, front view.
PDB entry 4kug coloured by chain, side view.
PDB entry 4kug coloured by chain, top view.
Source organism: Clostridium butyricum E4 str. BoNT E BL5262
Entry authors: Kim EJ, Kim S, Kim KJ

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxybutyryl-CoA dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 290 amino acids
Theoretical weight: 31.54 KDa
Source organism: Clostridium butyricum E4 str. BoNT E BL5262
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IEM5 (Residues: 1-282; Coverage: 100%)
Gene names: CLP_3850, hbd
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: R3
Unit cell:
a: 148.45Å b: 148.45Å c: 201.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.218 0.282
Expression system: Escherichia coli

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