› 4kvn

X-ray diffraction
3.1Å resolution

Crystal structure of Fab 39.29 in complex with Influenza Hemagglutinin A/Perth/16/2009 (H3N2)

Released:
DOI: 10.2210/pdb4kvn/pdb
PDB entry 4kvn coloured by chain, front view.
PDB entry 4kvn coloured by chain, side view.
PDB entry 4kvn coloured by chain, top view.
Source organisms:
Primary publication:
An in vivo human-plasmablast enrichment technique allows rapid identification of therapeutic influenza A antibodies.
Nakamura G, Chai N, Park S, Chiang N, Lin Z, Chiu H, Fong R, Yan D, Kim J, Zhang J, Lee WP, Estevez A, Coons M, Xu M, Lupardus P, Balazs M, Swem LR
Cell Host Microbe 14 93-103 (2013)
PMID: 23870317

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207732 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 505 amino acids
Theoretical weight: 56.69 KDa
Source organism: Influenza A virus (A/Perth/16/2009(H3N2))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: C6KNH7 (Residues: 25-520; Coverage: 88%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Human IgG Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Human IgG Light Chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 5 x NAG
Ligand PO4 1 x PO4
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I213
Unit cell:
a: 204.37Å b: 204.37Å c: 204.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.248
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

45 review citations

Development of therapeutic antibodies for the treatment of diseases.
Lu et al. (2020)
44 more

17 mentions without citation

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus.
Wu et al. (2017)
16 more