› 4kwe

X-ray diffraction
2.91Å resolution

GDP-bound, double-stranded, curved FtsZ protofilament structure

Released:
DOI: 10.2210/pdb4kwe/pdb
PDB entry 4kwe coloured by chain, front view.
PDB entry 4kwe coloured by chain, side view.
PDB entry 4kwe coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation.
Li Y, Hsin J, Zhao L, Cheng Y, Shang W, Huang KC, Wang HW, Ye S
Science 341 392-5 (2013)
PMID: 23888039

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 382 amino acids
Theoretical weight: 39.07 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN95 (Residues: 1-379; Coverage: 100%)
Gene names: MTCY270.18, Rv2150c, ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 3 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6422
Unit cell:
a: 132.509Å b: 132.509Å c: 321.549Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.241 0.267
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Splitsville: structural and functional insights into the dynamic bacterial Z ring.
Haeusser et al. (2016)
20 more

10 mentions without citation

FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation.
Li et al. (2013)
9 more