› 4l8o

X-ray diffraction
2.2Å resolution

Crystal structure of a bile-acid 7-alpha dehydratase (CLOHYLEM_06634) from Clostridium hylemonae DSM 15053 at 2.20 A resolution

Released:
DOI: 10.2210/pdb4l8o/pdb
PDB entry 4l8o coloured by chain, front view.
PDB entry 4l8o coloured by chain, side view.
PDB entry 4l8o coloured by chain, top view.
Source organism: [Clostridium] hylemonae DSM 15053
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
7-alpha,12-alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12-alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110164 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SnoaL-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 22.22 KDa
Source organism: [Clostridium] hylemonae DSM 15053
Expression system: Escherichia coli
UniProt:
  • Canonical: B4YSU1 (Residues: 1-169; Coverage: 100%)
Gene names: CLOHYLEM_06634, baiE
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

4 bound ligands:
Ligand CO 1 x CO
Ligand NA 1 x NA
Ligand SO4 1 x SO4
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R32
Unit cell:
a: 52.956Å b: 52.956Å c: 428.627Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.225
Expression system: Escherichia coli

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