› 4ld7

X-ray diffraction
2.83Å resolution

Crystal structure of AnaPT from Neosartorya fischeri

Released:
DOI: 10.2210/pdb4ld7/pdb
PDB entry 4ld7 coloured by chain, front view.
PDB entry 4ld7 coloured by chain, side view.
PDB entry 4ld7 coloured by chain, top view.
Source organism: Aspergillus fischeri
Primary publication:
Catalytic mechanism of stereospecific formation of cis-configured prenylated pyrroloindoline diketopiperazines by indole prenyltransferases.
Yu X, Zocher G, Xie X, Liebhold M, Schütz S, Stehle T, Li SM
Chem Biol 20 1492-501 (2013)
PMID: 24239009

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole diterpene prenyltransferase anaPT Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 445 amino acids
Theoretical weight: 49.93 KDa
Source organism: Aspergillus fischeri
Expression system: Escherichia coli
UniProt:
  • Canonical: A1DN10 (Residues: 1-437; Coverage: 100%)
Gene names: NFIA_055300, anaPT
Sequence domains: Tryptophan dimethylallyltransferase

Ligands and Environments

2 bound ligands:
Ligand PIS 16 x PIS
Ligand NA 16 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 97.55Å b: 242.59Å c: 145.32Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.203 0.203 0.202
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Prenyltransferases as key enzymes in primary and secondary metabolism.
Winkelblech et al. (2015)
4 more

4 mentions without citation

Data Science-Driven Analysis of Substrate-Permissive Diketopiperazine Reverse Prenyltransferase NotF: Applications in Protein Engineering and Cascade Biocatalytic Synthesis of (-)-Eurotiumin A.
Kelly et al. (2022)
3 more