› 4leh

X-ray diffraction
2.9Å resolution

Crystal structure of a bile-acid 7-alpha dehydratase (CLOSCI_03134) from Clostridium scindens ATCC 35704 at 2.90 A resolution

Released:
DOI: 10.2210/pdb4leh/pdb
PDB entry 4leh coloured by chain, front view.
PDB entry 4leh coloured by chain, side view.
PDB entry 4leh coloured by chain, top view.
Source organism: [Clostridium] scindens ATCC 35704
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
7-alpha,12-alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12-alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SnoaL-like domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 185 amino acids
Theoretical weight: 22.34 KDa
Source organism: [Clostridium] scindens ATCC 35704
Expression system: Escherichia coli
UniProt:
  • Canonical: B0NI18 (Residues: 4-169; Coverage: 98%)
Gene names: CLOSCI_03134, baiE
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 8 x SO4
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3121
Unit cell:
a: 109.322Å b: 109.322Å c: 118.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli

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