› 4lfg

X-ray diffraction
1.76Å resolution

Crystal structure of geranylgeranyl diphosphate synthase sub1274 (target efi-509455) from streptococcus uberis 0140j with bound magnesium and isopentyl diphosphate, fully liganded complex;

Released:
DOI: 10.2210/pdb4lfg/pdb
PDB entry 4lfg coloured by chain, front view.
PDB entry 4lfg coloured by chain, side view.
PDB entry 4lfg coloured by chain, top view.
Source organism: Streptococcus uberis 0140J
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Imker HJ, Al Obaidi N, Stead M, Love J, Poulter CD, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.3 KDa
Source organism: Streptococcus uberis 0140J
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9DUT7 (Residues: 1-289; Coverage: 100%)
Gene names: SUB1274, fps
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand IPE 4 x IPE
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 70.385Å b: 74.326Å c: 124.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.184
Expression system: Escherichia coli BL21(DE3)

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