› 4lg0

X-ray diffraction
2.19Å resolution

Structure of a ternary FOXO1-ETS1 DNA complex

Released:
DOI: 10.2210/pdb4lg0/pdb
PDB entry 4lg0 coloured by chain, front view.
PDB entry 4lg0 coloured by chain, side view.
PDB entry 4lg0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Birrane G, Choy WC, Datta D, Geiger CA, Grant MA

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117787 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Forkhead box protein O1 Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12778 (Residues: 143-270; Coverage: 20%)
Gene names: FKHR, FOXO1, FOXO1A
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Protein C-ets-1 Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 13.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14921 (Residues: 331-440; Coverage: 25%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*DTP*DTP*DCP*DAP*DCP*DGP*DGP*DTP*DTP*DTP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DTP*DTP*DGP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
DNA (5'-D(*DAP*DAP*DAP*DCP*DAP*DAP*DTP*DAP*DAP*DCP*DAP*DGP*DGP*DAP*DAP*DAP*DCP*DCP*DGP*DTP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.48 KDa

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 68.658Å b: 114.944Å c: 136.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.243
Expression system: Escherichia coli BL21(DE3)

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