› 4lq0

X-ray diffraction
2.68Å resolution

Crystal structure of the I-LtrWI LAGLIDADG homing endonuclease bound to target DNA.

Released:
DOI: 10.2210/pdb4lq0/pdb
PDB entry 4lq0 coloured by chain, front view.
PDB entry 4lq0 coloured by chain, side view.
PDB entry 4lq0 coloured by chain, top view.
Source organism: Leptographium truncatum
Entry authors: Chik J, Shen B, Stoddard B

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117795 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 35.16 KDa
Source organism: Leptographium truncatum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E0YCK3 (Residues: 1-304; Coverage: 100%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
top strand DNA target Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: Leptographium truncatum
Expression system: Not provided
bottom strand DNA target Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: Leptographium truncatum
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand PEG 1 x PEG
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 40.553Å b: 71.959Å c: 168.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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