› 4lqc

X-ray diffraction
2.3Å resolution

The crystal structures of the Brucella protein TcpB and the TLR adaptor protein TIRAP show structural differences in microbial TIR mimicry.

Released:
DOI: 10.2210/pdb4lqc/pdb
PDB entry 4lqc coloured by chain, front view.
PDB entry 4lqc coloured by chain, side view.
PDB entry 4lqc coloured by chain, top view.
Source organism: Brucella melitensis
Primary publication:
Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry.
Snyder GA, Deredge D, Waldhuber A, Fresquez T, Wilkins DZ, Smith PT, Durr S, Cirl C, Jiang J, Jennings W, Luchetti T, Snyder N, Sundberg EJ, Wintrode P, Miethke T, Xiao TS
J Biol Chem 289 669-79 (2014)
PMID: 24275656

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable 2' cyclic ADP-D-ribose synthase TcpB Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.88 KDa
Source organism: Brucella melitensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YF53 (Residues: 113-250; Coverage: 55%)
Gene names: BMEI1674, btp1, btpA, tcpB
Sequence domains: TIR domain
Structure domains: Toll/interleukin-1 receptor homology (TIR) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 67.45Å b: 67.3Å c: 60.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.263
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Assembly and localization of Toll-like receptor signalling complexes.
Gay et al. (2014)
17 more

6 mentions without citation

The Architecture of the TIR Domain Signalosome in the Toll-like Receptor-4 Signaling Pathway.
Guven-Maiorov et al. (2015)
5 more