› 4lt6

X-ray diffraction
2.79Å resolution

Crystal Structure of human poly(A) polymerase gamma

Released:
DOI: 10.2210/pdb4lt6/pdb
PDB entry 4lt6 coloured by chain, front view.
PDB entry 4lt6 coloured by chain, side view.
PDB entry 4lt6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human poly(A) polymerase gamma reveals a conserved catalytic core for canonical poly(A) polymerases.
Yang Q, Nausch LW, Martin G, Keller W, Doublié S
J Mol Biol 426 43-50 (2014)
PMID: 24076191

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 514 amino acids
Theoretical weight: 59.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BWT3 (Residues: 1-508; Coverage: 69%)
Gene names: PAP2, PAPG, PAPOLG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 3AT 2 x 3AT
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 68.87Å b: 89.92Å c: 201.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.257
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Therapeutic Potential and Biological Applications of Cordycepin and Metabolic Mechanisms in Cordycepin-Producing Fungi.
Qin et al. (2019)
3 more

3 mentions without citation

Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.
Whiteley et al. (2019)
2 more