› 4lx4

X-ray diffraction
1.56Å resolution

Crystal Structure Determination of Pseudomonas stutzeri endoglucanase Cel5A using a Twinned Data Set

Released:
DOI: 10.2210/pdb4lx4/pdb
PDB entry 4lx4 coloured by chain, front view.
PDB entry 4lx4 coloured by chain, side view.
PDB entry 4lx4 coloured by chain, top view.
Source organism: Pseudomonas stutzeri A1501
Primary publication:
Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.
Dutoit R, Delsaute M, Collet L, Vander Wauven C, Van Elder D, Berlemont R, Richel A, Galleni M, Bauvois C
Acta Crystallogr D Struct Biol 75 605-615 (2019)
PMID: 31205022

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.22 KDa
Source organism: Pseudomonas stutzeri A1501
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4VME5 (Residues: 31-360; Coverage: 100%)
Gene name: PST_2494
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 70.39Å b: 82.9Å c: 104.74Å
α: 90° β: 92.08° γ: 90°
R-values:
R R work R free
0.224 0.22 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Complete genome sequencing and comparative genome characterization of the lignocellulosic biomass degrading bacterium Pseudomonas stutzeri MP4687 from cattle rumen.
Patel et al. (2020)
3 more

2 mentions without citation

Isolation and Characterization of an Acidic, Salt-Tolerant Endoglucanase Cel5A from a Bacterial Strain Martelella endophytica YC6887 Genome.
Khan et al. (2021)
1 more