› 4m16

X-ray diffraction
1.85Å resolution

Crystal Structure of the N-terminal Fic Domain of Bartonella effector protein (Bep); substrate of VirB T4SS (VirB-translocated Bep effector protein) from Bartonella sp. AR 15-3

Released:
DOI: 10.2210/pdb4m16/pdb
PDB entry 4m16 coloured by chain, front view.
PDB entry 4m16 coloured by chain, side view.
PDB entry 4m16 coloured by chain, top view.
Source organism: Bartonella sp. AR 15-3
Primary publication:
Evolutionary Diversification of Host-Targeted Bartonella Effectors Proteins Derived from a Conserved FicTA Toxin-Antitoxin Module.
Schirmer T, de Beer TAP, Tamegger S, Harms A, Dietz N, Dranow DM, Edwards TE, Myler PJ, Phan I, Dehio C
Microorganisms 9 (2021)
PMID: 34442725

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 28.14 KDa
Source organism: Bartonella sp. AR 15-3
Expression system: Escherichia coli
UniProt:
  • Canonical: E6YQQ1 (Residues: 9-240; Coverage: 43%)
Gene names: BAR153v2_007450, BAR15_120228
Sequence domains: Fic/DOC family
Structure domains: Fido-like domain

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 40.24Å b: 47.48Å c: 67.97Å
α: 90° β: 105.64° γ: 90°
R-values:
R R work R free
0.166 0.164 0.211
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Biology and evolution of bacterial toxin-antitoxin systems.
Jurėnas et al. (2022)
3 more

1 mention without citation

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats.
Dou et al. (2022)