› 4m2p

X-ray diffraction
1.45Å resolution

Crystal structure of a non-myristoylated C39D recoverin mutant with one calcium ion bound to EF-hand 3

Released:
DOI: 10.2210/pdb4m2p/pdb
PDB entry 4m2p coloured by chain, front view.
PDB entry 4m2p coloured by chain, side view.
PDB entry 4m2p coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A highly conserved cysteine of neuronal calcium-sensing proteins controls cooperative binding of Ca2+ to recoverin.
Ranaghan MJ, Kumar RP, Chakrabarti KS, Buosi V, Kern D, Oprian DD
J Biol Chem 288 36160-7 (2013)
PMID: 24189072

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recoverin Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.25 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21457 (Residues: 2-202; Coverage: 100%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4
Unit cell:
a: 84.22Å b: 84.22Å c: 59.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biological chemistry and functionality of protein sulfenic acids and related thiol modifications.
Devarie-Baez et al. (2016)
2 more

3 mentions without citation

A highly conserved cysteine of neuronal calcium-sensing proteins controls cooperative binding of Ca2+ to recoverin.
Ranaghan et al. (2013)
2 more