› 4m63

X-ray diffraction
2.75Å resolution

Crystal Structure of a Filament-Like Actin Trimer Bound to the Bacterial Effector VopL

Released:
DOI: 10.2210/pdb4m63/pdb
PDB entry 4m63 coloured by chain, front view.
PDB entry 4m63 coloured by chain, side view.
PDB entry 4m63 coloured by chain, top view.
Source organisms:
Primary publication:
The bacterial effector VopL organizes actin into filament-like structures.
Zahm JA, Padrick SB, Chen Z, Pak CW, Yunus AA, Henry L, Tomchick DR, Chen Z, Rosen MK
Cell 155 423-34 (2013)
PMID: 24120140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145611 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
T3SS2 effector VopL nucleation of actin polymerization Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 26.82 KDa
Source organism: Vibrio parahaemolyticus BB22OP
Expression system: Escherichia coli BL21(DE3)
Structure domains:
Actin-5C Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 377 amino acids
Theoretical weight: 41.81 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 1-376, 1-286, 288-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 3 x ATP
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 287.759Å b: 66.084Å c: 103.16Å
α: 90° β: 94.01° γ: 90°
R-values:
R R work R free
0.222 0.219 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda

Quick links

Citations

6 review citations

The WH2 Domain and Actin Nucleation: Necessary but Insufficient.
Dominguez R. (2016)
5 more

4 mentions without citation

Actinous enigma or enigmatic actin: Folding, structure, and functions of the most abundant eukaryotic protein.
Povarova et al. (2014)
3 more