› 4m8b

X-ray diffraction
2.61Å resolution

Fungal Protein

Released:
DOI: 10.2210/pdb4m8b/pdb
PDB entry 4m8b coloured by chain, front view.
PDB entry 4m8b coloured by chain, side view.
PDB entry 4m8b coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of a new DNA-binding domain which regulates pathogenesis in a wide variety of fungi.
Lohse MB, Rosenberg OS, Cox JS, Stroud RM, Finer-Moore JS, Johnson AD
Proc Natl Acad Sci U S A 111 10404-10 (2014)
PMID: 24994900

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117831 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Uncharacterized protein YHR177W Chain: R
Molecule details ›
Chain: R
Length: 202 amino acids
Theoretical weight: 23.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38867 (Residues: 6-201; Coverage: 43%)
Gene name: YHR177W
Sequence domains: Gti1/Pac2 family
Chain A of dsDNA containing the cis-regulatory element Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
Chain B of dsDNA containing the cis-regulatory element Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 90.293Å b: 103.401Å c: 73.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 citation in other articles

Transcription Factors Encoded on Core and Accessory Chromosomes of Fusarium oxysporum Induce Expression of Effector Genes.
van der Does et al. (2016)
11 more

2 mentions without citation

Structure of a new DNA-binding domain which regulates pathogenesis in a wide variety of fungi.
Lohse et al. (2014)
1 more