› 4mdc

X-ray diffraction
1.78Å resolution

Crystal structure of glutathione S-transferase from Sinorhizobium meliloti 1021, NYSGRC target 021389

Released:
DOI: 10.2210/pdb4mdc/pdb
PDB entry 4mdc coloured by chain, front view.
PDB entry 4mdc coloured by chain, side view.
PDB entry 4mdc coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Shabalin IG, Bacal P, Cooper DR, Stead M, Ahmed M, Hammonds J, Bonanno J, Seidel R, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187925 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 252 amino acids
Theoretical weight: 29.48 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92SP3 (Residues: 1-230; Coverage: 100%)
Gene names: SMc00407, gst4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 182.181Å b: 53.508Å c: 111.123Å
α: 90° β: 115.88° γ: 90°
R-values:
R R work R free
0.163 0.161 0.19
Expression system: Escherichia coli BL21(DE3)

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