› 4mdh

X-ray diffraction
2.5Å resolution

REFINED CRYSTAL STRUCTURE OF CYTOPLASMIC MALATE DEHYDROGENASE AT 2.5-ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb4mdh/pdb
PDB entry 4mdh coloured by chain, front view.
PDB entry 4mdh coloured by chain, side view.
PDB entry 4mdh coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Refined crystal structure of cytoplasmic malate dehydrogenase at 2.5-A resolution.
Birktoft JJ, Rhodes G, Banaszak LJ
Biochemistry 28 6065-81 (1989)
PMID: 2775751

Function and Biology Details

Reactions catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD(+) = 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 36.39 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P11708 (Residues: 2-334; Coverage: 100%)
Gene name: MDH1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 139.2Å b: 86.6Å c: 58.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided

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Citations

1 review citation

Malate dehydrogenase: a model for structure, evolution, and catalysis.
Goward et al. (1994)
68 other articles

15 mentions without citation

Toward a resolution of the introns early/late debate: only phase zero introns are correlated with the structure of ancient proteins.
de Souza et al. (1998)
14 more