› 4mjw

X-ray diffraction
1.95Å resolution

Crystal Structure of Choline Oxidase in Complex with the Reaction Product Glycine Betaine

Released:
DOI: 10.2210/pdb4mjw/pdb
PDB entry 4mjw coloured by chain, front view.
PDB entry 4mjw coloured by chain, side view.
PDB entry 4mjw coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Structure of choline oxidase in complex with the reaction product glycine betaine.
Salvi F, Wang YF, Weber IT, Gadda G
Acta Crystallogr D Biol Crystallogr 70 405-13 (2014)
PMID: 24531474

Function and Biology Details

Reaction catalysed: 
(1a) choline + O(2) = betaine aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 59.9 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7X2H8 (Residues: 1-546; Coverage: 100%)
Gene name: codA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
Ligand BET 2 x BET
Ligand ACT 2 x ACT
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 87.35Å b: 87.35Å c: 353.484Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.181
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Spatial and Temporal Profile of Glycine Betaine Accumulation in Plants Under Abiotic Stresses.
Annunziata et al. (2019)
1 more

4 mentions without citation

Crystal Structure of Alcohol Oxidase from Pichia pastoris.
Koch et al. (2016)
3 more