› 4mpi

X-ray diffraction
1.6Å resolution

Crystal structure of the chitin-binding module (CBM18) of a chitinase-like protein from Hevea brasiliensis

Released:
DOI: 10.2210/pdb4mpi/pdb
PDB entry 4mpi coloured by chain, front view.
PDB entry 4mpi coloured by chain, side view.
PDB entry 4mpi coloured by chain, top view.
Source organism: Hevea brasiliensis subsp. brasiliensis
Primary publication:
Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
Martínez-Caballero S, Cano-Sánchez P, Mares-Mejía I, Díaz-Sánchez AG, Macías-Rubalcava ML, Hermoso JA, Rodríguez-Romero A
FEBS J 281 4535-54 (2014)
PMID: 25104038

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable inactive chitinase-like protein LaCIC Chains: A, B
Molecule details ›
Chains: A, B
Length: 45 amino acids
Theoretical weight: 4.51 KDa
Source organism: Hevea brasiliensis subsp. brasiliensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GUD7 (Residues: 1-43; Coverage: 15%)
Gene name: LACIC
Sequence domains: Chitin recognition protein
Structure domains: Endochitinase-like

Ligands and Environments

2 bound ligands:
Ligand MES 1 x MES
Ligand DIO 1 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 38.515Å b: 38.515Å c: 100.934Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.179 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chitin and chitinase: Role in pathogenicity, allergenicity and health.
Patel et al. (2017)
2 more

3 mentions without citation

Chitinase: diversity, limitations, and trends in engineering for suitable applications.
Oyeleye et al. (2018)
2 more