› 4mtn

X-ray diffraction
2.58Å resolution

Crystal structure of transcription termination factor NusA from Planctomyces limnophilus DSM 3776

Released:
DOI: 10.2210/pdb4mtn/pdb
PDB entry 4mtn coloured by chain, front view.
PDB entry 4mtn coloured by chain, side view.
PDB entry 4mtn coloured by chain, top view.
Source organism: Planctopirus limnophila DSM 3776
Entry authors: Chang C, Hatzos-Skintges C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 46.03 KDa
Source organism: Planctopirus limnophila DSM 3776
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5SQ09 (Residues: 2-405; Coverage: 91%)
Gene names: Plim_2559, nusA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 162.026Å b: 54.065Å c: 62.859Å
α: 90° β: 110.69° γ: 90°
R-values:
R R work R free
0.224 0.222 0.251
Expression system: Escherichia coli BL21(DE3)

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