› 4mxw

X-ray diffraction
3.6Å resolution

Structure of heterotrimeric lymphotoxin LTa1b2 bound to lymphotoxin beta receptor LTbR and anti-LTa Fab

Released:
DOI: 10.2210/pdb4mxw/pdb
PDB entry 4mxw coloured by chain, front view.
PDB entry 4mxw coloured by chain, side view.
PDB entry 4mxw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dimerization of LTβR by LTα1β2 is necessary and sufficient for signal transduction.
Sudhamsu J, Yin J, Chiang EY, Starovasnik MA, Grogan JL, Hymowitz SG
Proc Natl Acad Sci U S A 110 19896-901 (2013)
PMID: 24248355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-208415 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Tumor necrosis factor receptor superfamily member 3 Chains: R, S
Molecule details ›
Chains: R, S
Length: 193 amino acids
Theoretical weight: 21.37 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P36941 (Residues: 41-211; Coverage: 42%)
Gene names: D12S370, LTBR, TNFCR, TNFR3, TNFRSF3
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2
Lymphotoxin-alpha Chains: A, X
Molecule details ›
Chains: A, X
Length: 157 amino acids
Theoretical weight: 17.31 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01374 (Residues: 62-205; Coverage: 84%)
Gene names: LTA, TNFB, TNFSF1
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Lymphotoxin-beta Chains: B, D, Y, Z
Molecule details ›
Chains: B, D, Y, Z
Length: 210 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q06643 (Residues: 86-244; Coverage: 65%)
Gene names: LTB, TNFC, TNFSF3
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
anti-Lymphotoxin alpha antibody heavy chain Chains: H, W
Molecule details ›
Chains: H, W
Length: 213 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
anti-Lymphotoxin alpha antibody light chain Chains: L, V
Molecule details ›
Chains: L, V
Length: 211 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 211.201Å b: 52.428Å c: 253.241Å
α: 90° β: 101.1° γ: 90°
R-values:
R R work R free
0.234 0.231 0.296
Expression systems:
  • Trichoplusia ni
  • Cricetulus griseus

Quick links

Citations

7 review citations

Structural principles of tumor necrosis factor superfamily signaling.
Vanamee et al. (2018)
6 more

7 mentions without citation

Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab.
Chin et al. (2018)
6 more