› 4mz7

X-ray diffraction
1.8Å resolution

Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase

Released:
DOI: 10.2210/pdb4mz7/pdb
PDB entry 4mz7 coloured by chain, front view.
PDB entry 4mz7 coloured by chain, side view.
PDB entry 4mz7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase.
Zhu C, Gao W, Zhao K, Qin X, Zhang Y, Peng X, Zhang L, Dong Y, Zhang W, Li P, Wei W, Gong Y, Yu XF
Nat Commun 4 2722 (2013)
PMID: 24217394

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 539 amino acids
Theoretical weight: 62.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 109-626; Coverage: 83%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand DGT 5 x DGT
Ligand MG 2 x MG
Ligand DTP 1 x DTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 147.404Å b: 103.608Å c: 92.134Å
α: 90° β: 121.98° γ: 90°
R-values:
R R work R free
0.196 0.194 0.226
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Regulation of deoxynucleotide metabolism in cancer: novel mechanisms and therapeutic implications.
Kohnken et al. (2015)
12 more

11 mentions without citation

SAMHD1 Functions and Human Diseases.
Coggins et al. (2020)
10 more