› 4mze

X-ray diffraction
1.8Å resolution

Crystal structure of hPIV3 hemagglutinin-neuraminidase, H552Q/Q559R mutant

Released:
DOI: 10.2210/pdb4mze/pdb
PDB entry 4mze coloured by chain, front view.
PDB entry 4mze coloured by chain, side view.
PDB entry 4mze coloured by chain, top view.
Source organism: Human parainfluenza 3 virus (strain NIH 47885)
Primary publication:
Interaction between the hemagglutinin-neuraminidase and fusion glycoproteins of human parainfluenza virus type III regulates viral growth in vivo.
Xu R, Palmer SG, Porotto M, Palermo LM, Niewiesk S, Wilson IA, Moscona A
mBio 4 e00803-13 (2013)
PMID: 24149514

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Hemagglutinin-neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 48.82 KDa
Source organism: Human parainfluenza 3 virus (strain NIH 47885)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08492 (Residues: 136-572; Coverage: 76%)
Gene name: HN
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
6 bound ligands:
Ligand NAG 2 x NAG
Ligand PO4 3 x PO4
Ligand CA 2 x CA
Ligand EDO 15 x EDO
Ligand PEG 4 x PEG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 84.029Å b: 96.643Å c: 105.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.196
Expression system: Trichoplusia ni

Quick links

Citations

6 review citations

Measles Virus Fusion Protein: Structure, Function and Inhibition.
Plattet et al. (2016)
5 more

2 mentions without citation

Interaction between the hemagglutinin-neuraminidase and fusion glycoproteins of human parainfluenza virus type III regulates viral growth in vivo.
Xu et al. (2013)
1 more