› 4nd1

X-ray diffraction
2.15Å resolution

Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with cofactor (b-nicotinamide adenine dinucleotide) and inhibitor (oxamic acid)

Released:
DOI: 10.2210/pdb4nd1/pdb
PDB entry 4nd1 coloured by chain, front view.
PDB entry 4nd1 coloured by chain, side view.
PDB entry 4nd1 coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.
Cook WJ, Senkovich O, Hernandez A, Speed H, Chattopadhyay D
Int J Biol Macromol 74 608-19 (2015)
PMID: 25542170

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 33.88 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CYZ2 (Residues: 17-337; Coverage: 95%)
Gene name: cgd7_480
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand OXM 2 x OXM
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3221
Unit cell:
a: 94.816Å b: 94.816Å c: 185.072Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.236
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Metabolic Signatures of Cryptosporidiumparvum-Infected HCT-8 Cells and Impact of Selected Metabolic Inhibitors on C. parvum Infection under Physioxia and Hyperoxia.
Vélez et al. (2021)
7 more

2 mentions without citation

Polyphenolic Profile of Herniaria hemistemon Aerial Parts Extract and Assessment of Its Anti-Cryptosporidiosis in a Murine Model: In Silico Supported In Vivo Study.
Ghareeb et al. (2023)
1 more