› 4nd3

X-ray diffraction
2.05Å resolution

Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (l-lactic acid) and cofactor (b-nicotinamide adenine dinucleotide)

Released:
DOI: 10.2210/pdb4nd3/pdb
PDB entry 4nd3 coloured by chain, front view.
PDB entry 4nd3 coloured by chain, side view.
PDB entry 4nd3 coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Biochemical and structural characterization of Cryptosporidium parvum Lactate dehydrogenase.
Cook WJ, Senkovich O, Hernandez A, Speed H, Chattopadhyay D
Int J Biol Macromol 74 608-19 (2015)
PMID: 25542170

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 33.88 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CYZ2 (Residues: 17-337; Coverage: 95%)
Gene name: cgd7_480
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand 2OP 1 x 2OP
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 95.454Å b: 95.454Å c: 186.025Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.241
Expression system: Escherichia coli

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Citations

10 citation in other articles

Metabolic Signatures of Cryptosporidiumparvum-Infected HCT-8 Cells and Impact of Selected Metabolic Inhibitors on C. parvum Infection under Physioxia and Hyperoxia.
Vélez et al. (2021)
9 more