› 4neh

X-ray diffraction
2.75Å resolution

An internal ligand-bound, metastable state of a leukocyte integrin, aXb2

Released:
DOI: 10.2210/pdb4neh/pdb
PDB entry 4neh coloured by chain, front view.
PDB entry 4neh coloured by chain, side view.
PDB entry 4neh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An internal ligand-bound, metastable state of a leukocyte integrin, αXβ2.
Sen M, Yuki K, Springer TA
J Cell Biol 203 629-42 (2013)
PMID: 24385486

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138498 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Integrin alpha-X Chain: A
Molecule details ›
Chain: A
Length: 1094 amino acids
Theoretical weight: 120.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P20702 (Residues: 20-1101; Coverage: 95%)
Gene names: CD11C, ITGAX
Sequence domains:
Structure domains:
Integrin beta-2 Chain: B
Molecule details ›
Chain: B
Length: 686 amino acids
Theoretical weight: 75.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05107 (Residues: 23-696; Coverage: 90%)
Gene names: CD18, ITGB2, MFI7
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand CA 5 x CA
Ligand MG 2 x MG
Ligand NAG 1 x NAG
Ligand CL 1 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 126.967Å b: 131.444Å c: 190.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.225
Expression system: Homo sapiens

Quick links

Citations

18 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
17 more

23 mentions without citation

Molecular Modeling Insights into the Structure and Behavior of Integrins: A Review.
Tvaroška et al. (2023)
22 more