› 4nf9

X-ray diffraction
2.8Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185544 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinetochore scaffold 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NG31 (Residues: 2117-2337; Coverage: 9%)
Gene names: CASC5, KIAA1570, KNL1
Sequence domains: Knl1 RWD C-terminal domain
Kinetochore-associated protein NSL1 homolog Chains: C, D
Molecule details ›
Chains: C, D
Length: 26 amino acids
Theoretical weight: 3.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96IY1 (Residues: 256-281; Coverage: 9%)
Gene names: C1orf48, DC31, DC8, MIS14, NSL1

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 60.71Å b: 71.63Å c: 177.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

Joined at the hip: kinetochores, microtubules, and spindle assembly checkpoint signaling.
Sacristan et al. (2015)
17 more

4 mentions without citation

The dynamics of signal amplification by macromolecular assemblies for the control of chromosome segregation.
Lee et al. (2014)
3 more