› 4nos

X-ray diffraction
2.25Å resolution

HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE WITH INHIBITOR

Released:
DOI: 10.2210/pdb4nos/pdb
PDB entry 4nos coloured by chain, front view.
PDB entry 4nos coloured by chain, side view.
PDB entry 4nos coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of nitric oxide synthase isoforms reveals striking active-site conservation.
Fischmann TO, Hruza A, Niu XD, Fossetta JD, Lunn CA, Dolphin E, Prongay AJ, Reichert P, Lundell DJ, Narula SK, Weber PC
Nat Struct Biol 6 233-42 (1999)
PMID: 10074942

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 427 amino acids
Theoretical weight: 49.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35228 (Residues: 82-508; Coverage: 37%)
Gene names: NOS2, NOS2A
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
4 bound ligands:
Ligand ZN 2 x ZN
Ligand H2B 1 x H2B
Ligand ITU 4 x ITU
Ligand H4B 3 x H4B
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 89.759Å b: 156.672Å c: 190.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.289
Expression system: Escherichia coli

Quick links

Citations

52 review citations

Nitric oxide synthases: structure, function and inhibition.
Alderton et al. (2001)
51 more

39 mentions without citation

Development of nitric oxide synthase inhibitors for neurodegeneration and neuropathic pain.
Mukherjee et al. (2014)
38 more