› 4npj

X-ray diffraction
2.1Å resolution

Extended-Synaptotagmin 2, C2A- and C2B-domains

Released:
DOI: 10.2210/pdb4npj/pdb
PDB entry 4npj coloured by chain, front view.
PDB entry 4npj coloured by chain, side view.
PDB entry 4npj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and Ca²⁺-binding properties of the tandem C₂ domains of E-Syt2.
Xu J, Bacaj T, Zhou A, Tomchick DR, Südhof TC, Rizo J
Structure 22 269-80 (2014)
PMID: 24373768

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-106072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extended synaptotagmin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0FGR8 (Residues: 363-660; Coverage: 32%)
Gene names: ESYT2, FAM62B, KIAA1228
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand NA 1 x NA
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 62.3Å b: 62.3Å c: 226.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
7 more

3 mentions without citation

Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
Brault et al. (2019)
2 more