› 4nqa

X-ray diffraction
3.1Å resolution

Crystal structure of liganded hRXR-alpha/hLXR-beta heterodimer on DNA

Released:
DOI: 10.2210/pdb4nqa/pdb
PDB entry 4nqa coloured by chain, front view.
PDB entry 4nqa coloured by chain, side view.
PDB entry 4nqa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the retinoid X receptor α-liver X receptor β (RXRα-LXRβ) heterodimer on DNA.
Lou X, Toresson G, Benod C, Suh JH, Philips KJ, Webb P, Gustafsson JA
Nat Struct Mol Biol 21 277-81 (2014)
PMID: 24561505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117888 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Retinoic acid receptor RXR-alpha Chains: A, H
Molecule details ›
Chains: A, H
Length: 365 amino acids
Theoretical weight: 41.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 98-462; Coverage: 79%)
Gene names: NR2B1, RXRA
Sequence domains:
Structure domains:
Oxysterols receptor LXR-beta Chains: B, I
Molecule details ›
Chains: B, I
Length: 391 amino acids
Theoretical weight: 44.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55055 (Residues: 72-175, 176-460; Coverage: 85%)
Gene names: LXRB, NER, NR1H2, UNR
Sequence domains:
Structure domains:
Nuclear receptor coactivator 2 Chains: C, D, J, K
Molecule details ›
Chains: C, D, J, K
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15596 (Residues: 686-698; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
5'-D(*TP*AP*AP*GP*GP*TP*CP*AP*CP*TP*TP*CP*AP*GP*GP*TP*CP*A)-3' Chains: E, L
Molecule details ›
Chains: E, L
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
5'-D(*TP*AP*TP*GP*AP*CP*CP*TP*GP*AP*AP*GP*TP*GP*AP*CP*CP*T)-3' Chains: F, M
Molecule details ›
Chains: F, M
Length: 18 nucleotides
Theoretical weight: 5.52 KDa

Ligands and Environments

3 bound ligands:
Ligand 9CR 2 x 9CR
Ligand ZN 8 x ZN
Ligand 965 2 x 965
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43
Unit cell:
a: 85.783Å b: 85.783Å c: 238.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.18 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Nuclear Receptors, RXR, and the Big Bang.
Evans et al. (2014)
13 more

22 mentions without citation

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
21 more