› 4ns4

X-ray diffraction
2.15Å resolution

Crystal structure of cold-active estarase from Psychrobacter cryohalolentis K5T

Released:
DOI: 10.2210/pdb4ns4/pdb
PDB entry 4ns4 coloured by chain, front view.
PDB entry 4ns4 coloured by chain, side view.
PDB entry 4ns4 coloured by chain, top view.
Source organism: Psychrobacter cryohalolentis K5
Entry authors: Boyko KM, Petrovskaya LE, Gorbacheva MA, Korgenevsky DA, Novototskaya-Vlasova KA, Rivkina EM, Dolgikh DA, Kirpichnikov MP, Lipkin AV, Popov VO

Function and Biology Details

Reaction catalysed: 
An acetic ester + H(2)O = an alcohol + acetate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.66 KDa
Source organism: Psychrobacter cryohalolentis K5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1QEU6 (Residues: 1-315; Coverage: 100%)
Gene name: Pcryo_0023
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: P212121
Unit cell:
a: 53.2Å b: 59.4Å c: 105.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.254
Expression system: Escherichia coli

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