› 4nsw

X-ray diffraction
2.2Å resolution

Crystal structure of the BAR-PH domain of ACAP1

Released:
DOI: 10.2210/pdb4nsw/pdb
PDB entry 4nsw coloured by chain, front view.
PDB entry 4nsw coloured by chain, side view.
PDB entry 4nsw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature.
Pang X, Fan J, Zhang Y, Zhang K, Gao B, Ma J, Li J, Deng Y, Zhou Q, Egelman EH, Hsu VW, Sun F
Dev Cell 31 73-86 (2014)
PMID: 25284369

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 43.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15027 (Residues: 1-377; Coverage: 51%)
Gene names: ACAP1, CENTB1, KIAA0050
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A, PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 42.46Å b: 59.72Å c: 168.95Å
α: 90° β: 91.4° γ: 90°
R-values:
R R work R free
0.214 0.211 0.266
Expression system: Escherichia coli

Quick links

Citations

3 review citations

To degrade or not to degrade: mechanisms and significance of endocytic recycling.
Cullen et al. (2018)
2 more

6 mentions without citation

How curvature-generating proteins build scaffolds on membrane nanotubes.
Simunovic et al. (2016)
5 more