› 4o1k

X-ray diffraction
1.83Å resolution

Crystal structures of two tetrameric beta-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora.

Released:
DOI: 10.2210/pdb4o1k/pdb
PDB entry 4o1k coloured by chain, front view.
PDB entry 4o1k coloured by chain, side view.
PDB entry 4o1k coloured by chain, top view.
Source organism: Sordaria macrospora
Primary publication:
Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora.
Lehneck R, Neumann P, Vullo D, Elleuche S, Supuran CT, Ficner R, Pöggeler S
FEBS J 281 1759-72 (2014)
PMID: 24506675

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.95 KDa
Source organism: Sordaria macrospora
Expression system: Escherichia coli
UniProt:
  • Canonical: C1L336 (Residues: 61-284; Coverage: 79%)
Gene names: SMACR_03614, cas2
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: F222
Unit cell:
a: 83.2Å b: 94.11Å c: 96.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.211
Expression system: Escherichia coli

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Citations

4 review citations

An overview of the alpha-, beta- and gamma-carbonic anhydrases from Bacteria: can bacterial carbonic anhydrases shed new light on evolution of bacteria?
Capasso et al. (2015)
3 more

2 mentions without citation

Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora.
Lehneck et al. (2014)
1 more