› 4o65

X-ray diffraction
1.8Å resolution

Crystal structure of the cupredoxin domain of amoB from Nitrosocaldus yellowstonii

Released:
DOI: 10.2210/pdb4o65/pdb
PDB entry 4o65 coloured by chain, front view.
PDB entry 4o65 coloured by chain, side view.
PDB entry 4o65 coloured by chain, top view.
Source organism: Candidatus Nitrosocaldus yellowstonensis
Primary publication:
Structural conservation of the B subunit in the ammonia monooxygenase/particulate methane monooxygenase superfamily.
Lawton TJ, Ham J, Sun T, Rosenzweig AC
Proteins 82 2263-7 (2014)
PMID: 24523098

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative archaeal ammonia monooxygenase subunit B Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.16 KDa
Source organism: Candidatus Nitrosocaldus yellowstonensis
Expression system: Escherichia coli
UniProt:
  • Canonical: B0LKZ4 (Residues: 31-186; Coverage: 75%)
Gene name: amoB
Sequence domains: Monooxygenase subunit B protein
Structure domains: Particulate methane monooxygenase, b subunit. Chain: A, domain 1

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P43212
Unit cell:
a: 47.886Å b: 47.886Å c: 192.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.229
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Enzymatic oxidation of methane.
Sirajuddin et al. (2015)
6 more

2 mentions without citation

Structural conservation of the B subunit in the ammonia monooxygenase/particulate methane monooxygenase superfamily.
Lawton et al. (2014)
1 more