› 4o68

X-ray diffraction
2.44Å resolution

Structure of human cyclic GMP-AMP synthase (cGAS)

Released:
DOI: 10.2210/pdb4o68/pdb
PDB entry 4o68 coloured by chain, front view.
PDB entry 4o68 coloured by chain, side view.
PDB entry 4o68 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop.
Zhang X, Wu J, Du F, Xu H, Sun L, Chen Z, Brautigam CA, Zhang X, Chen ZJ
Cell Rep 6 421-30 (2014)
PMID: 24462292

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N884 (Residues: 147-522; Coverage: 72%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 162.194Å b: 47.051Å c: 58.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.18 0.244
Expression system: Escherichia coli

Quick links

Citations

123 review citations

Regulation and function of the cGAS-STING pathway of cytosolic DNA sensing.
Chen et al. (2016)
122 more

7 mentions without citation

The cGAS-STING pathway as a therapeutic target in inflammatory diseases.
Decout et al. (2021)
6 more