› 4o8m

X-ray diffraction
1.7Å resolution

Crystal structure of a trap periplasmic solute binding protein actinobacillus succinogenes 130z, target EFI-510004, with bound L-galactonate

Released:
DOI: 10.2210/pdb4o8m/pdb
PDB entry 4o8m coloured by chain, front view.
PDB entry 4o8m coloured by chain, side view.
PDB entry 4o8m coloured by chain, top view.
Source organism: Actinobacillus succinogenes 130Z
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP dicarboxylate transporter, DctP subunit Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 346 amino acids
Theoretical weight: 38.36 KDa
Source organism: Actinobacillus succinogenes 130Z
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6VKP1 (Residues: 1-328; Coverage: 100%)
Gene name: Asuc_0158
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand SO4 16 x SO4
Ligand 2Q2 8 x 2Q2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 97.233Å b: 143.593Å c: 97.458Å
α: 90° β: 99.49° γ: 90°
R-values:
R R work R free
0.191 0.188 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
12 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)