› 4ogc

X-ray diffraction
2.8Å resolution

Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii

Released:
DOI: 10.2210/pdb4ogc/pdb
PDB entry 4ogc coloured by chain, front view.
PDB entry 4ogc coloured by chain, side view.
PDB entry 4ogc coloured by chain, top view.
Source organism: Actinomyces naeslundii str. Howell 279
Primary publication:
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Science 343 1247997 (2014)
PMID: 24505130

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 1101 amino acids
Theoretical weight: 124.9 KDa
Source organism: Actinomyces naeslundii str. Howell 279
Expression system: Escherichia coli
UniProt:
  • Canonical: J3F2B0 (Residues: 1-1101; Coverage: 100%)
Gene names: HMPREF1129_2620, cas9
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand MN 2 x MN
Ligand MG 2 x MG
Ligand SPD 1 x SPD
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1, ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 74.61Å b: 132.56Å c: 80.04Å
α: 90° β: 95.38° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli

Quick links

Citations

230 review citations

Development and applications of CRISPR-Cas9 for genome engineering.
Hsu et al. (2014)
229 more

7 mentions without citation

Annotation and Classification of CRISPR-Cas Systems.
Makarova et al. (2015)
6 more