› 4ohv

X-ray diffraction
2.3Å resolution

C. Elegans Clp1 bound to AMP-PNP, and Mg2+

Released:
DOI: 10.2210/pdb4ohv/pdb
PDB entry 4ohv coloured by chain, front view.
PDB entry 4ohv coloured by chain, side view.
PDB entry 4ohv coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.
Dikfidan A, Loll B, Zeymer C, Magler I, Clausen T, Meinhart A
Mol Cell 54 975-86 (2014)
PMID: 24813946

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein clpf-1 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 48.36 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52874 (Residues: 1-428; Coverage: 100%)
Gene names: F59A2.4, clpf-1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
Ligand 2PE 1 x 2PE
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P31
Unit cell:
a: 100.72Å b: 100.72Å c: 40.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

CLP1 as a novel player in linking tRNA splicing to neurodegenerative disorders.
Weitzer et al. (2015)
1 more

2 mentions without citation

Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex.
Hayne et al. (2022)
1 more