› 4oo8

X-ray diffraction
2.5Å resolution

Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA

Released:
DOI: 10.2210/pdb4oo8/pdb
PDB entry 4oo8 coloured by chain, front view.
PDB entry 4oo8 coloured by chain, side view.
PDB entry 4oo8 coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Crystal structure of Cas9 in complex with guide RNA and target DNA.
Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O
Cell 156 935-49 (2014)
PMID: 24529477

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117927 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1372 amino acids
Theoretical weight: 158.96 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA (5'-D(*CP*CP*AP*GP*CP*CP*AP*AP*GP*CP*GP*CP*AP*CP*CP*TP*AP*AP*TP*TP*TP*CP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 23 nucleotides
Theoretical weight: 6.93 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Not provided
RNA (97-MER) Chains: B, E
Molecule details ›
Chains: B, E
Length: 98 nucleotides
Theoretical weight: 31.69 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P1
Unit cell:
a: 76.713Å b: 105.694Å c: 126.817Å
α: 97.68° β: 98.43° γ: 100.31°
R-values:
R R work R free
0.224 0.222 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

362 review citations

Development and applications of CRISPR-Cas9 for genome engineering.
Hsu et al. (2014)
361 more

78 mentions without citation

Editing the epigenome: technologies for programmable transcription and epigenetic modulation.
Thakore et al. (2016)
77 more