› 4oqr

X-ray diffraction
1.81Å resolution

Structure of a CYP105AS1 mutant in complex with compactin

Released:
DOI: 10.2210/pdb4oqr/pdb
PDB entry 4oqr coloured by chain, front view.
PDB entry 4oqr coloured by chain, side view.
PDB entry 4oqr coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Primary publication:
Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum.
McLean KJ, Hans M, Meijrink B, van Scheppingen WB, Vollebregt A, Tee KL, van der Laan JM, Leys D, Munro AW, van den Berg MA
Proc Natl Acad Sci U S A 112 2847-52 (2015)
PMID: 25691737

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase CYP105AS1 Chain: A
Molecule details ›
Chain: A
Length: 457 amino acids
Theoretical weight: 50.63 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
UniProt:
  • Canonical: W5VH56 (Residues: 1-401; Coverage: 100%)
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand 2UO 1 x 2UO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 39.36Å b: 86.81Å c: 139.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Growing a circular economy with fungal biotechnology: a white paper.
Meyer et al. (2020)
17 more

6 mentions without citation

Recent Structural Insights into Cytochrome P450 Function.
Guengerich et al. (2016)
5 more