› 4oww

X-ray diffraction
2.3Å resolution

Structural basis of SOSS1 in complex with a 35nt ssDNA

Released:
DOI: 10.2210/pdb4oww/pdb
PDB entry 4oww coloured by chain, front view.
PDB entry 4oww coloured by chain, side view.
PDB entry 4oww coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of SOSS1 complex assembly and recognition of ssDNA.
Ren W, Chen H, Sun Q, Tang X, Lim SC, Huang J, Song H
Cell Rep 6 982-991 (2014)
PMID: 24630995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117953 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
Integrator complex subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68E01 (Residues: 1-501; Coverage: 48%)
Gene names: C1orf193, C1orf60, INTS3
Sequence domains: Integrator complex subunit 3 N-terminal
SOSS complex subunit B1 Chain: B
Molecule details ›
Chain: B
Length: 211 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BQ15 (Residues: 1-211; Coverage: 100%)
Gene names: LP3587, NABP2, OBFC2B, SSB1
Sequence domains: Single-stranded DNA binding protein Ssb-like, OB fold
Structure domains: Nucleic acid-binding proteins
SOSS complex subunit C Chain: C
Molecule details ›
Chain: C
Length: 104 amino acids
Theoretical weight: 11.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRY2 (Residues: 1-104; Coverage: 100%)
Gene names: C9orf80, HSPC043, HSPC291, INIP, SSBIP1
Sequence domains: SOSS complex subunit C
DNA (5'-D(P*TP*TP*TP*TP*TP*T)-3') Chain: L
Molecule details ›
Chain: L
Length: 35 nucleotides
Theoretical weight: 10.6 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 82.35Å b: 90.97Å c: 111.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Replication protein A and more: single-stranded DNA-binding proteins in eukaryotic cells.
Liu et al. (2016)
2 more

2 mentions without citation

Genomic regulation of transcription and RNA processing by the multitasking Integrator complex.
Welsh et al. (2023)
1 more