› 4oyl

X-ray diffraction
2.05Å resolution

Humicola insolens cutinase in complex with mono-ethylphosphate

Released:
DOI: 10.2210/pdb4oyl/pdb
PDB entry 4oyl coloured by chain, front view.
PDB entry 4oyl coloured by chain, side view.
PDB entry 4oyl coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Thermodynamic and structural investigation of the specific SDS binding of Humicola insolens cutinase.
Kold D, Dauter Z, Laustsen AK, Brzozowski AM, Turkenburg JP, Nielsen AD, Koldsø H, Petersen E, Schiøtt B, De Maria L, Wilson KS, Svendsen A, Wimmer R
Protein Sci 23 1023-35 (2014)
PMID: 24832484

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 194 amino acids
Theoretical weight: 20.41 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A075B5G4 (Residues: 1-194; Coverage: 100%)
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MIR 3 x MIR
Ligand OYL 3 x OYL

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 71.63Å b: 66.4Å c: 71.98Å
α: 90° β: 119.3° γ: 90°
R-values:
R R work R free
0.146 0.143 0.184
Expression system: Aspergillus oryzae

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Citations

2 review citations

Enzyme catalyzes ester bond synthesis and hydrolysis: The key step for sustainable usage of plastics.
Lai et al. (2022)
1 more

8 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
7 more