› 4p2s

X-ray diffraction
1.94Å resolution

Alanine Scanning Mutagenesis Identifies an Asparagine-Arginine-Lysine Triad Essential to Assembly of the Shell of the Pdu Microcompartment

Released:
DOI: 10.2210/pdb4p2s/pdb
PDB entry 4p2s coloured by chain, front view.
PDB entry 4p2s coloured by chain, side view.
PDB entry 4p2s coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Saintpaul str. SARA26
Primary publication:
Alanine scanning mutagenesis identifies an asparagine-arginine-lysine triad essential to assembly of the shell of the Pdu microcompartment.
Sinha S, Cheng S, Sung YW, McNamara DE, Sawaya MR, Yeates TO, Bobik TA
J Mol Biol 426 2328-45 (2014)
PMID: 24747050

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative propanediol utilization protein PduA Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 101 amino acids
Theoretical weight: 10.51 KDa
Source organism: Salmonella enterica subsp. enterica serovar Saintpaul str. SARA26
Expression system: Escherichia coli BL21(DE3)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 7 x GOL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 183.156Å b: 105.402Å c: 67.269Å
α: 90° β: 94.84° γ: 90°
R-values:
R R work R free
0.194 0.194 0.209
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Bacterial microcompartments and the modular construction of microbial metabolism.
Kerfeld et al. (2015)
4 more

1 mention without citation

Alanine scanning mutagenesis identifies an asparagine-arginine-lysine triad essential to assembly of the shell of the Pdu microcompartment.
Sinha et al. (2014)