› 4p6q

X-ray diffraction
2Å resolution

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA Recognition Motifs

Released:
DOI: 10.2210/pdb4p6q/pdb
PDB entry 4p6q coloured by chain, front view.
PDB entry 4p6q coloured by chain, side view.
PDB entry 4p6q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Arieti F, Gabus C, Tambalo M, Huet T, Round A, Thore S
Nucleic Acids Res 42 6742-52 (2014)
PMID: 24748666

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Msx2-interacting protein Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96T58 (Residues: 335-620; Coverage: 8%)
Gene names: KIAA0929, MINT, SHARP, SPEN
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 60.67Å b: 69.71Å c: 88.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

15 review citations

Long Non-coding RNA Structure and Function: Is There a Link?
Zampetaki et al. (2018)
14 more

2 mentions without citation

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Arieti et al. (2014)
1 more