› 4p96

X-ray diffraction
2.2Å resolution

FadR, Fatty Acid Responsive Transcription Factor from Vibrio cholerae

Released:
DOI: 10.2210/pdb4p96/pdb
PDB entry 4p96 coloured by chain, front view.
PDB entry 4p96 coloured by chain, side view.
PDB entry 4p96 coloured by chain, top view.
Source organism: Vibrio cholerae HC-21A1
Primary publication:
The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand.
Shi W, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ
Nat Commun 6 6032 (2015)
PMID: 25607896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid metabolism regulator protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 32.03 KDa
Source organism: Vibrio cholerae HC-21A1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UKZ1 (Residues: 1-279; Coverage: 100%)
Gene names: VCHC21A1_1913, fadR
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41
Unit cell:
a: 87.48Å b: 87.48Å c: 81.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Allosteric control of transcription in GntR family of transcription regulators: A structural overview.
Jain D. (2015)
3 more

1 mention without citation

The 40-residue insertion in Vibrio cholerae FadR facilitates binding of an additional fatty acyl-CoA ligand.
Shi et al. (2015)