› 4pcv

X-ray diffraction
2.05Å resolution

The structure of BdcA (YjgI) from E. coli

Released:
DOI: 10.2210/pdb4pcv/pdb
PDB entry 4pcv coloured by chain, front view.
PDB entry 4pcv coloured by chain, side view.
PDB entry 4pcv coloured by chain, top view.
Source organism: Escherichia coli P0299438.9
Primary publication:
BdcA, a protein important for Escherichia coli biofilm dispersal, is a short-chain dehydrogenase/reductase that binds specifically to NADPH.
Lord DM, Baran AU, Wood TK, Peti W, Page R
PLoS One 9 e105751 (2014)
PMID: 25244619

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BdcA (YjgI) Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 24.88 KDa
Source organism: Escherichia coli P0299438.9
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X1Z4 (Residues: 3-239, 1-240; Coverage: 100%)
Gene name: ECP02994389_4551
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand PGE 4 x PGE
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 131.058Å b: 52.461Å c: 69.807Å
α: 90° β: 118.06° γ: 90°
R-values:
R R work R free
0.202 0.201 0.222
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Approaches to Dispersing Medical Biofilms.
Fleming et al. (2017)
2 more

2 mentions without citation

BdcA, a protein important for Escherichia coli biofilm dispersal, is a short-chain dehydrogenase/reductase that binds specifically to NADPH.
Lord et al. (2014)
1 more