› 4peg

X-ray diffraction
2Å resolution

Dbr1 in complex with guanosine-5'-monophosphate

Released:
DOI: 10.2210/pdb4peg/pdb
PDB entry 4peg coloured by chain, front view.
PDB entry 4peg coloured by chain, side view.
PDB entry 4peg coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1.
Montemayor EJ, Katolik A, Clark NE, Taylor AB, Schuermann JP, Combs DJ, Johnsson R, Holloway SP, Stevens SW, Damha MJ, Hart PJ
Nucleic Acids Res 42 10845-55 (2014)
PMID: 25123664

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lariat debranching enzyme Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 360 amino acids
Theoretical weight: 41.91 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene names: DBR1, EHI_062730
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand MN 5 x MN
Ligand 5GP 5 x 5GP
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 72.831Å b: 141.499Å c: 212.942Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.229
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Metallophosphoesterases: structural fidelity with functional promiscuity.
Matange et al. (2015)
6 more

5 mentions without citation

Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1.
Montemayor et al. (2014)
4 more